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Crosslink Effect and Albumin Adsorption onto Chitosan/Alginate Multilayered Systems: An in situ QCM‐D Study
Author(s) -
Martins Gabriela V.,
Merino Esther G.,
Mano João F.,
Alves Natália M.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000193
Subject(s) - adsorption , chitosan , polyelectrolyte , quartz crystal microbalance , protein adsorption , adhesion , chemical engineering , human serum albumin , polymer , chemistry , surface forces apparatus , in situ , layer by layer , polymer chemistry , layer (electronics) , materials science , nanotechnology , chromatography , organic chemistry , engineering
The adsorption of HSA onto CHI/ALG multilayer assemblies was assessed in situ using QCM‐D. It was found that the behavior of HSA on biomaterials surface can be tuned by adjusting parameters of the polyelectrolyte system such as pH, layer number, crosslinker and polymer terminal layer. Our results confirmed the key role of electrostatic interactions during HSA adsorption, since oppositely charged surfaces were more effective in promoting protein adhesion. QCM‐D data revealed that crosslinking (CHI/ALG) 5 CHI films allows HSA to become adsorbed in physiological conditions. Our results suggested that the biological potential of biopolymers and the mild conditions of the LbL technique turn these natural nanoassemblies into a suitable choice to be used as pH‐sensitive coatings.