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Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds
Author(s) -
Guillemette Maxime D.,
Park Hyoungshin,
Hsiao James C.,
Jain Saloni R.,
Larson Benjamin L.,
Langer Robert,
Freed Lisa E.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000165
Subject(s) - elastomer , tissue engineering , materials science , c2c12 , elongation , biomedical engineering , scanning electron microscope , polymer , ultimate tensile strength , myocyte , composite material , myogenesis , microbiology and biotechnology , biology , medicine
Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.