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Multifunctional Hybrid Three‐dimensionally Woven Scaffolds for Cartilage Tissue Engineering
Author(s) -
Moutos Franklin T.,
Estes Bradley T.,
Guilak Farshid
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000124
Subject(s) - chondrogenesis , tissue engineering , biomedical engineering , cartilage , scaffold , extracellular matrix , progenitor cell , biomaterial , materials science , chemistry , stem cell , nanotechnology , microbiology and biotechnology , anatomy , biology , engineering , biochemistry
The successful replacement of large‐scale cartilage defects or osteoarthritic lesions using tissue‐engineering approaches will likely require composite biomaterial scaffolds that have biomimetic mechanical properties and can provide cell‐instructive cues to control the growth and differentiation of embedded stem or progenitor cells. This study describes a novel method of constructing multifunctional scaffolds for cartilage tissue engineering that can provide both mechanical support and biological stimulation to seeded progenitor cells. 3‐D woven PCL scaffolds were infiltrated with a slurry of homogenized CDM of porcine origin, seeded with human ASCs, and cultured for up to 42 d under standard growth conditions. These constructs were compared to scaffolds derived solely from CDM as well as 3‐D woven PCL fabric without CDM. While all scaffolds promoted a chondrogenic phenotype of the ASCs, CDM scaffolds showed low compressive and shear moduli and contracted significantly during culture. Fiber‐reinforced CDM scaffolds and 3‐D woven PCL scaffolds maintained their mechanical properties throughout the culture period, while supporting the accumulation of a cartilaginous extracellular matrix. These findings show that fiber‐reinforced hybrid scaffolds can be produced with biomimetic mechanical properties as well as the ability to promote ASC differentiation and chondrogenesis in vitro.