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The Softer and More Hydrophobic the Better: Influence of the Side Chain of Polymethacrylate Nanoparticles for Cellular Uptake
Author(s) -
Lorenz Steffen,
Hauser Christoph P.,
Autenrieth Benjamin,
Weiss Clemens K.,
Landfester Katharina,
Mailänder Volker
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000099
Subject(s) - chemistry , nanoparticle , side chain , biophysics , polymer chemistry , chemical engineering , polymer , nanotechnology , organic chemistry , materials science , engineering , biology
Intracellular uptake of nanoparticles is highly interesting for labeling of cells, drug delivery, or non‐viral gene delivery. In this study we have synthesized a wide variety of poly(alkyl methacrylate) nanoparticles with the same size and investigated their uptake into cells. The nanoparticles were prepared from alkylmethacrylates with different linear and branched ester chains as well as from benzylmethacrylate using the miniemulsion polymerizaiton technique. By adding a fluorescent dye as a marker, the internalization of the nanoparticles could be investigated quantitatively with flow cytometry and qualitatively with confocal laser scanning microscopy. With increasing side chain of the ester and therefore increasing hydrophobicity and at glass transition temperature ( T g ), below the incubation temperature of 37 °C the uptake of the nanoparticles into cells is favored.