Premium
Synthesis of Linear Polyamines with Different Amine Spacings and their Ability to Form dsDNA/siRNA Complexes Suitable for Transfection
Author(s) -
Fischer Wiebke,
Brissault Blandine,
Prévost Sylvain,
Kopaczynska Marta,
Andreou Ioanna,
Janosch Andrea,
Gradzielski Michael,
Haag Rainer
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000082
Subject(s) - chemistry , dna , transfection , imine , polyamine , amine gas treating , polymer , crystallography , polymer chemistry , stereochemistry , organic chemistry , biochemistry , gene , catalysis
In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to phosphorus (N/P) ratios using small‐angle neutron scattering (SANS) and atomic force microscopy (AFM) techniques. AFM images showed that while linear poly(ethylene imine) (PEI)/DNA complex results in bigger spherical aggregates, poly(propylene imine)s forms torroid and cigar shaped structures upon complexation with DNA. The poly(butylene imine)s (LPBI)s form compact and soluble DNA complexes with a radii range of R g = 15–30 nm. Among the studied linear polyamines, the LPBIs did show the best transfection efficiency.