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Evaluation of the Information Content of Sedimentation Equilibrium Data in Self‐Interacting Systems
Author(s) -
Ang Shirley,
Rowe Arthur J
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000065
Subject(s) - virial coefficient , virial theorem , sedimentation equilibrium , sedimentation , statistical physics , noise (video) , interference (communication) , content (measure theory) , thermodynamics , physics , chemistry , mathematics , computer science , mathematical analysis , chromatography , geology , ultracentrifuge , quantum mechanics , artificial intelligence , computer network , channel (broadcasting) , sediment , galaxy , image (mathematics) , paleontology
Abstract Fitting r = f(c) as opposed to the usual c = f(r) to the inverted form of the sedimentation equilibrium equation for interacting solute (INVEQ algorithm), it is shown by detailed simulation and by experimentation that stable, simultaneous estimates can be retrieved for both virial (2nd BM/3rd CM) and specific interaction ( K a ) terms. In suitable systems estimates for two distinct second virial (BM) and single K a terms can equally be defined. Whilst cell loading level is critical, noise level in the interference fringe data is shown to have surprisingly little influence on these outcomes.