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Charge and Size Dependence of Liposome Diffusion in Semidilute Biopolymer Solutions
Author(s) -
Rusu Laura,
Lumma Dirk,
Rädler Joachim O.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000033
Subject(s) - diffusion , surface charge , chemistry , liposome , biopolymer , hydrodynamic radius , particle (ecology) , particle size , chemical physics , colloid , electrophoresis , pulmonary surfactant , polymer , biophysics , analytical chemistry (journal) , chromatography , thermodynamics , organic chemistry , biochemistry , physics , oceanography , biology , copolymer , geology
The diffusion of liposomes and PL/DNA complexes in mucin and collagen solutions, considered to model ‘in vivo’ colloidal gene delivery vector transport, is studied with FCS. The diffusion of defined liposomes is investigated as a function of particle size, surface charge, and the deviation from the Stokes‐Einstein behavior. In all cases the self‐diffusion coefficient decreases exponentially with polymer concentration. The same surface charge dependence of diffusion is observed in mucin for PL/DNA complexes with either positive or negative excess charge. Incubation of positively‐charged PL/DNA complexes in a natural lung surfactant lipid increases the diffusion coefficients to almost the same as for the negatively‐charged PL/DNA complexes.