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A New Approach for in vitro Imaging of Breast Cancer Cells by Anti‐Metadherin Targeted PVA‐Pyrene
Author(s) -
Medine E. Ilker,
Odaci Dilek,
Gacal Bahadir N.,
Gacal Burcin,
Sakarya Serhan,
Unak Perihan,
Timur Suna,
Yagci Yusuf
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201000009
Subject(s) - vinyl alcohol , pyrene , fluorescence microscope , in vitro , chemistry , fluorescence , breast cancer , biophysics , click chemistry , molecule , cell culture , cancer cell , antibody , nanotechnology , cancer , materials science , biochemistry , polymer chemistry , polymer , organic chemistry , biology , immunology , physics , quantum mechanics , genetics
Poly(vinyl alcohol)‐pyrene‐anti‐metadherin (PVA‐Py‐(Anti‐MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA‐Py prepared by “Click chemistry” approach is targeted by Anti‐MTDH antibody and applied to the MCF‐7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non‐targeted structure (PVA‐Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.