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Reductive Degradation Behavior of Bioreducible Poly(disulfide amine) for Enhancing SiRNA Efficiency
Author(s) -
Kim Sun Hwa,
Ou Mei,
Bull David A.,
Kim Sung Wan
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900482
Subject(s) - degradation (telecommunications) , amine gas treating , disulfide bond , chemistry , combinatorial chemistry , chemical engineering , organic chemistry , biochemistry , computer science , engineering , telecommunications
Bioreducible cationic polymer poly(CBA‐DAH) containing repeated disulfide linkages on the polymer backbone was synthesized through Michael‐type polyadditions of CBA to DAH monomers. Poly(CBA‐DAH) could spontaneously form nanoscale polyelectrolyte complexes through electrostatic interactions with siRNA in an aqueous phase. These nanoparticles were rapidly degraded under the reductive cytoplasmic environment with subsequently releasing the siRNA cargo into the cytoplasm where RNAi takes place, as a result of the breakdown of disulfide bonds in the polymers. The reductive degradation behavior of the poly(CBA‐DAH)/siRNA polyplexes is more likely to increase RNAi activity with enhancing the cytoplasmic localization of siRNA molecules. Poly(CBA‐DAH) may have great potential as a gene carrier especially for therapeutic applications of siRNAs owing to the reductive degradation characteristics.