Premium
Orientation Control of Photo‐Immobilized Antibodies on the Surface of Azobenzene‐Containing Polymers by the Introduction of Functional Groups
Author(s) -
Mouri Makoto,
Ikawa Taiji,
Narita Mamiko,
Hoshino Fumihiko,
Watanabe Osamu
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900394
Subject(s) - azobenzene , adsorption , orientation (vector space) , polymer , materials science , chemistry , chemical engineering , surface (topology) , polymer chemistry , nanotechnology , composite material , organic chemistry , geometry , mathematics , engineering
Abstract In our photo‐induced immobilization technique for an antibody (IgG) using azopolymers, the introduction of COOH and NMe 2 into the azopolymers, which can introduce surface charges, strongly affected the immobilization properties such as the efficiency of immobilization and the activity of the immobilized IgG (i.e., the orientation of the immobilized IgG). The introduction of COOH promoted a more active orientation of the immobilized IgG. The orientation was determined during the adsorption process onto the azopolymer surface in solution before photo‐immobilization, and was maintained during the photo‐immobilization. The surface charge of the azopolymer appears to be an important factor for IgG orientation, which involves electrostatic interactions between its Fab and the azopolymer surface.