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DNA Polyplexes Formed Using PEGylated Biodegradable Hyperbranched Polymers
Author(s) -
Tao Lei,
Chou William C.,
Tan Beng H.,
Davis Thomas P.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900378
Subject(s) - polymer , oligomer , polymer chemistry , peg ratio , chemistry , zeta potential , materials science , chemical engineering , organic chemistry , nanoparticle , nanotechnology , finance , engineering , economics
Abstract A novel PEGylated biodegradable hyperbranched PEG‐ b ‐PDMAEMA has been synthesized. The low toxicity, small molecular weight PDMAEMA chains were crosslinked using a biodegradable disulfide‐based dimethacrylate (DSDMA) agent to yield higher molecular weight hyperbranched polymers. PEG chains were linked onto the polymer surface, masking the positive charge (as shown by Zeta potential measurements) and reducing the toxicity of the polymer. The hyperbranched structures were also cleaved under reducing conditions and analyzed, confirming the expected component structures. The hyperbranched polymer was mixed with DNA and efficient binding was shown to occur through electrostatic interactions. The hyperbranched structures could be reduced easily, generating lower toxicity oligomer chains.