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Nano‐Encapsulation of Lipase by Self‐Assembled Nanogels: Induction of High Enzyme Activity and Thermal Stabilization
Author(s) -
Sawada Shinichi,
Akiyoshi Kazunari
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900304
Subject(s) - nanogel , pullulan , thermostability , lipase , chemistry , thermal stability , immobilized enzyme , denaturation (fissile materials) , chemical engineering , colloid , enzyme , organic chemistry , polymer chemistry , chromatography , polysaccharide , nuclear chemistry , drug delivery , engineering
The effects of self‐assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially k cat , drastically increased in the presence of nanogels of cholesterol‐bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 °C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization‐induced aggregation. Nano‐encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme.