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Gelatin Hydrogel Prepared by Photo‐initiated Polymerization and Loaded with TGF‐ β 1 for Cartilage Tissue Engineering
Author(s) -
Hu Xiaohong,
Ma Lie,
Wang Chengchao,
Gao Changyou
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900275
Subject(s) - gelatin , self healing hydrogels , methacrylic acid , tissue engineering , polymerization , swelling , chondrocyte , dynamic mechanical analysis , polymer chemistry , chemical engineering , materials science , polymer , chemistry , biophysics , biomedical engineering , composite material , biochemistry , in vitro , medicine , engineering , biology
Abstract Gelatin is a nature‐derived protein having good cytocompatibility, and widely used in tissue engineering particularly in a form of a hydrogel. To obtain the hydrogel with good enough mechanical properties, however, measures are still need to be taken. In this work, the gelatin molecule was modified with methacrylic acid (MA) to obtain crosslinkable gelatin (GM), which formed a chemically crosslinked hydrogel by photoinitiating polymerization. The gelation time could be easily tuned and showed an inverse relationship with the GM concentration. After photo‐irradiation for 20 min there was no detectable double carbon bond in the hydrogen spectrum of high resolution magic angle spinning nuclear magnetic resonance spectroscopy ( 1 H HR‐MAS NMR). With the increase of the GM concentration, storage modulus and loss modulus of the hydrogels increased, but their swelling ratio and mesh size decreased. Weight loss of the hydrogels was also affected by the polymer concentration. Transform growth factor‐ β 1 (TGF‐ β 1) was incorporated into the GM hydrogel to improve its bioactivity. In vitro chondrocyte culture showed that the GM hydrogel had indeed good performance to support chondrocyte growth and maintain chondrocytic phenotype. Incorporation of TGF‐ β 1 could further improve the biological activity in terms of cell proliferation and extracellular matrix secretion.