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Novel Poly(amidoamine)s with Pendant Primary Amines as Highly Efficient Gene Delivery Vectors
Author(s) -
Liu Min,
Chen Jun,
Cheng YanPing,
Xue YaNan,
Zhuo RenXi,
Huang ShiWen
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900265
Subject(s) - amidoamine , poly(amidoamine) , polymer , polyelectrolyte , gene delivery , polymer chemistry , chemistry , transfection , dendrimer , cationic polymerization , michael reaction , organic chemistry , biochemistry , gene , catalysis
Three poly(amidoamine)s with pendant primary amines were synthesized by Michael polyaddition of a diamine to N,N‐ methylenebis(acrylamide). The poly(amidoamine)s with primary and tertiary amines possess a high buffer capacity between pH = 5 and 7, which facilitates the escape of polymer/DNA complexes from endosomes according to the ‘proton sponge hypothesis’. In vitro cytotoxicity of poly(amidoamine)s depends on the side‐chain structure of the polymer. Polymers 1a – 1c can condense plasmid DNA by electrostatic interactions to form nanosized polyelectrolyte complexes with a positive surface charge. In vitro transfection results indicate that the transfection efficiency of polymer 1b under optimized conditions is comparable to that of commercial branched PEI 25 000.