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Water Soluble Polymer Protected Lipofectamine 2000/DNA Complexes for Solid‐Phase Transfection
Author(s) -
Zhang Qiao,
Cheng SiXue,
Zhang XianZheng,
Zhuo RenXi
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900255
Subject(s) - transfection , lipofectamine , polymer , hela , chemistry , dna , microbiology and biotechnology , polymer chemistry , gene , in vitro , biochemistry , biology , organic chemistry , vector (molecular biology) , recombinant dna
A fast degrading cholic acid‐functionalized star poly( DL ‐lactide) has been used to fabricate polymer films to support Lipofectamine 2000/DNA complexes for mediating solid‐phase transfection. To improve the gene expression activity, a water‐soluble polymer, poly‐ α , β ‐[ N ‐(2‐hydroxyethyl)‐ L ‐aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in polymer films. The degradation of the polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.