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On the Effects of UV‐C and pH on the Mechanical Behavior, Molecular Conformation and Cell Viability of Collagen‐Based Scaffold for Vascular Tissue Engineering
Author(s) -
Achilli Matteo,
Lagueux Jean,
Mantovani Diego
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900248
Subject(s) - viability assay , chemistry , biophysics , scaffold , tissue engineering , cell adhesion , adhesion , substrate (aquarium) , cell , biomedical engineering , biochemistry , organic chemistry , biology , medicine , ecology
Collagen‐based vascular substitutes represent in VTE a valid alternative for the replacement of diseased small‐calibre blood vessels. In this study, collagen gel‐based scaffolds were crosslinked combining modulation of pH and UV‐C radiation. The effects on the mechanical properties, on the molecular structure and on cell viability and morphology were investigated. The mechanical response increased as a function of pH or UV‐C dose and strongly depended on the test speed. Collagen molecular conformation resulted only slightly modified. While cell adhesion was not significantly altered, cell proliferation partially decreased in function of pH and UV‐C. These findings suggest that UV‐C treated collagen gels can represent an adequate substrate for VTE applications.