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Functional L ‐Lysine Dendritic Macromolecules as Liver‐Imaging Probes
Author(s) -
Luo Kui,
Liu Gang,
Zhang Xiaowei,
She Wenchuan,
He Bin,
Nie Yu,
Li Li,
Wu Yao,
Zhang Zhirong,
Gong Qiyong,
Gao Fabao,
Song Bin,
Ai Hua,
Gu Zhongwei
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900231
Subject(s) - dendrimer , chemistry , in vitro , macromolecule , in vivo , hepatocyte , peptide , biophysics , cytotoxicity , biochemistry , biology , microbiology and biotechnology
Liver‐imaging probes are prepared through the conjugation of Gd chelates and galactosyl moieties to peptide dendrimers. The dendritic probes possessing highly controlled structures and a single molecular weight have a two‐fold increase in T 1 relaxivity to 9.1 × 10 3 (Gd M ) −1 s −1 compared to Gd‐DTPA. No obvious cytotoxicity of this multifunctional dendritic agent is discovered in vitro . The dendrimer bearing galactosyl moieties leads to a much‐higher hepatocyte‐cell uptake in vitro and provides good signal‐intensity enhancement (35%) of mouse liver in vivo especially at 60 min after intravenous injection. In comparison, non‐targeting Gd dendrimers provide only an 11% enhancement of imaging contrast at the same time point. Overall, the dendrimers bearing galactosyl moieties may be used as liver‐imaging probes.