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Cellular Evaluation of Synthesized Insulin/Transferrin Bioconjugates for Oral Insulin Delivery Using Intelligent Complexation Hydrogels
Author(s) -
Shofner Justin P.,
Phillips Margaret A.,
Peppas Nicholas A.
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900223
Subject(s) - conjugate , transferrin , insulin , chemistry , glucose transporter , self healing hydrogels , monolayer , permeability (electromagnetism) , bioavailability , biophysics , chromatography , biochemistry , endocrinology , membrane , pharmacology , polymer chemistry , biology , mathematical analysis , mathematics
Insulin transport across the epithelial cell layer in the small intestine was studied using insulin/transferrin conjugates with and without the presence of P(MAA ‐g‐ EG) microparticles in contact with a co‐culture of Caco‐2/HT29‐MTX cells. The insulin/transferrin conjugate was shown to increase transport relative to pure insulin by a factor of 7, achieving an apparent permeability of 37 × 10 9 cm · s −1 . The presence of P(MAA‐ g ‐EG) microparticles increased conjugate transport by a factor of 14 times relative to insulin, achieving an apparent permeability of 72.8 × 10 9 cm · s −1 . The presence of the microparticles in solution was found to improve conjugate transport by nearly 100% with little to no change in cell monolayer integrity.