Cellular Evaluation of Synthesized Insulin/Transferrin Bioconjugates for Oral Insulin Delivery Using Intelligent Complexation Hydrogels | Zendy

Shofner Justin P. | Zendy; Phillips Margaret A. | Zendy; Peppas Nicholas A. | Zendy

Premium

Cellular Evaluation of Synthesized Insulin/Transferrin Bioconjugates for Oral Insulin Delivery Using Intelligent Complexation Hydrogels

Author(s) -

Shofner Justin P.,

Phillips Margaret A.,

Peppas Nicholas A.

Publication year - 2010

Publication title -

macromolecular bioscience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.924

H-Index - 105

eISSN - 1616-5195

pISSN - 1616-5187

DOI - 10.1002/mabi.200900223

Subject(s) - conjugate , transferrin , insulin , chemistry , glucose transporter , self healing hydrogels , monolayer , permeability (electromagnetism) , bioavailability , biophysics , chromatography , biochemistry , endocrinology , membrane , pharmacology , polymer chemistry , biology , mathematical analysis , mathematics

Insulin transport across the epithelial cell layer in the small intestine was studied using insulin/transferrin conjugates with and without the presence of P(MAA ‐g‐ EG) microparticles in contact with a co‐culture of Caco‐2/HT29‐MTX cells. The insulin/transferrin conjugate was shown to increase transport relative to pure insulin by a factor of 7, achieving an apparent permeability of 37 × 10 9 cm · s −1 . The presence of P(MAA‐ g ‐EG) microparticles increased conjugate transport by a factor of 14 times relative to insulin, achieving an apparent permeability of 72.8 × 10 9 cm · s −1 . The presence of the microparticles in solution was found to improve conjugate transport by nearly 100% with little to no change in cell monolayer integrity.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research