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Poly(ethylene glycol)‐ block ‐Poly(glycidyl methacrylate) with Oligoamine Side Chains as Efficient Gene Vectors
Author(s) -
Ma Ming,
Li Feng,
Chen Fujie,
Cheng Sixue,
Zhuo Renxi
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900183
Subject(s) - ethylene glycol , glycidyl methacrylate , cationic polymerization , polymer chemistry , diethylenetriamine , atom transfer radical polymerization , triethylenetetramine , peg ratio , methacrylate , chemistry , side chain , polymer , copolymer , polymerization , materials science , organic chemistry , finance , economics
Well‐defined diblock copolymers, poly(ethylene glycol)‐ block ‐poly(glycidyl methacrylate)s (PEG‐ b ‐PGMAs), with different poly(glycidyl methacrylate) (PGMA) chains, were prepared via atom transfer radical polymerization (ATRP) from the same macromolecular initiator 2‐bromoisobutyryl‐terminated poly(ethylene glycol) (PEG). Ethyldiamine (EDA), diethylenetriamine (DETA), triethylenetetramine (TETA), and polyethyleneimine (PEI) with an $\overline M _{\rm w}$ of 400 (PEI 400 ) were used to decorate PEG‐ b ‐PGMAs to get the cationic polymers PEG‐ b ‐PGMA‐ oligoamines. These cationic polymers possessed high buffer capability and could condense plasmid DNA (pDNA) into nanoscaled complexes of 125–530 nm. These complexes showed the positive zeta potential of 20–35 mV at N/P ratios of 10–50. Most of them exhibited very low cytotoxicity and good transfection efficiency in 293T cells. The presence of the serum medium did not decrease the transfection efficiency due to the steric stabilization of the PEG chains.