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Layer‐by‐Layer Nano‐Encapsulation of Microbes: Controlled Cell Surface Modification and Investigation of Substrate Uptake in Bacteria
Author(s) -
Franz Bettina,
Balkundi Shantanu S.,
Dahl Christiane,
Lvov Yuri M.,
Prange Alexander
Publication year - 2010
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900142
Subject(s) - polyelectrolyte , sulfur , sulfide , bacteria , surface charge , coating , chemistry , chemical engineering , surface modification , nano , nanotechnology , biophysics , materials science , organic chemistry , polymer , biology , engineering , genetics
LbL nano self‐assembly coating of A. vinosum with different polyelectrolyte combinations is presented as an example to investigate substrate uptake in bacteria. The effects of surface charge and the formation of a physical barrier provides new insights in the contact mechanisms between the cell surface and insoluble elemental sulfur. Furthermore, uptake of sulfide by encapsulated cells was investigated. Growth experiments of coated cells showed that surface charge did neither affect sulfide uptake nor the contact formation between the cells and solid sulfur. However, increasing layers slowed or inhibited the uptake of sulfide and elemental sulfur. This work demonstrates how defining surface properties of bacteria has potential for microbiological and biotechnological applications.