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Surface Coating with a Thermoresponsive Copolymer for the Culture and Non‐Enzymatic Recovery of Mouse Embryonic Stem Cells
Author(s) -
Loh Xian Jun,
Gong Jiansheng,
Sakuragi Makoto,
Kitajima Takashi,
Liu Mingzhe,
Li Jun,
Ito Yoshihiro
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900081
Subject(s) - copolymer , gelatin , embryonic stem cell , stem cell , homogeneous , coating , tissue engineering , cell culture , chemistry , polymer chemistry , surface modification , poly(n isopropylacrylamide) , materials science , chemical engineering , biophysics , biochemistry , biomedical engineering , nanotechnology , microbiology and biotechnology , organic chemistry , polymer , biology , medicine , physics , thermodynamics , genetics , engineering , gene
A thermoresponsive substrate based on a triblock copolymer, poly( N ‐isopropylacrylamide)‐ block ‐poly[( R )‐3‐hydroxybutyrate]‐ block ‐poly( N ‐isopropylacrylamide) (PNIPAAm‐PHB‐PNIPAAm), co‐coated with gelatin, was developed for the culture and non‐enzymatic recovery of mouse embryonic stem cells. After culture, the cells could be detached by cooling at 4 °C for 20 min without trypsin digestion. The embryonic stem cells remained undifferentiated after culture on the gelatin/copolymer‐coated surfaces, similar to standard culture techniques. Overall, the triblock copolymer coating was superior to the PNIPAAm homopolymer coating in terms of supporting better cell growth, being more stable, presenting a more homogeneous surface coating, and maintaining pluripotency of the embryonic stem cells.
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