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Covalent Immobilization of Subtilisin A onto Thin Films of Maleic Anhydride Copolymers
Author(s) -
Tasso Mariana,
Cordeiro Ana L.,
Salchert Katrin,
Werner Carsten
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200900005
Subject(s) - biofouling , maleic anhydride , copolymer , subtilisin , immobilized enzyme , covalent bond , biopolymer , polymer chemistry , chemistry , fouling , chemical engineering , materials science , maleic acid , organic chemistry , enzyme , polymer , membrane , biochemistry , engineering
Enzymes cleaving the biopolymer adhesives of fouling organisms are attracting attention for the prevention of biofouling. We report a versatile and robust method to confine the serine protease Subtilisin A (or Subtilisin Carlsberg) to surfaces to be protected against biofouling. The approach consists of the covalent immobilization of the protease onto maleic anhydride copolymer thin film coatings. High‐swelling poly(ethylene‐ alt ‐maleic anhydride) (PEMA) copolymer layers permitted significantly higher enzyme loadings and activities than compact poly(octadecene‐ alt ‐maleic anhydride) (POMA) films. Substantial fractions of the immobilized, active enzyme layers were found to be conserved upon storage in deionized water for several hours. Ongoing studies explore the potentialities of the developed bioactive coatings to reduce the adhesion of various fouling organisms.