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Micropatterning of Covalently Attached Biotin on Poly(lactic acid) Film Surfaces
Author(s) -
Rasal Rahul M.,
Hirt Douglas E.
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800374
Subject(s) - micropatterning , carbodiimide , contact angle , covalent bond , attenuated total reflection , chemistry , fourier transform infrared spectroscopy , polymer chemistry , biotin , lactic acid , x ray photoelectron spectroscopy , streptavidin , fluorescence microscope , acrylic acid , chemical engineering , materials science , infrared spectroscopy , fluorescence , organic chemistry , nanotechnology , polymer , copolymer , biochemistry , optics , composite material , bacteria , engineering , biology , genetics , physics
Abstract A two‐step approach was used to micropattern covalently attached biotin on the surface of poly(lactic acid) (PLA) film. Poly(acrylic acid) (PAA) was micropatterned on PLA using photolithography in step 1, followed by carbodiimide wet chemistry to covalently attach biotin to acid groups in step 2. The PAA micropatterning reaction progression was monitored using attenuated total reflectance‐Fourier transform infrared (ATR‐FT‐IR) spectroscopy, water contact angle goniometry, and atomic force microscopy (AFM). The PAA‐biotin conjugation reaction characterization using XPS confirmed the carbodiimide mediated amidation reaction. The resultant PLA film was then immersed into a solution of fluorescence‐conjugated streptavidin and examined under fluorescence microscopy to reveal various micropatterns