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Disulfide‐Crosslinked Electrospun Poly( γ ‐glutamic acid) Nonwovens as Reduction‐Responsive Scaffolds
Author(s) -
Yoshida Hiroaki,
Klinkhammer Kristina,
Matsusaki Michiya,
Möller Martin,
Klee Doris,
Akashi Mitsuru
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800334
Subject(s) - cystamine , electrospinning , biocompatible material , tissue engineering , chemistry , disulfide bond , polymer chemistry , cell adhesion , adhesion , chemical engineering , fiber , synthetic fiber , in vitro , materials science , polymer , biomedical engineering , organic chemistry , biochemistry , medicine , engineering
Novel water‐insoluble, and reduction‐responsive nonwoven scaffolds were fabricated from γ ‐PGA and tested in cell culture. An electrospinning method was developed to produce scaffolds of fibers with diameters of 0.05–0.5 µm. Crosslinking of the fibers with cystamine in the presence of EDC resulted in water‐insoluble γ ‐PGA nonwovens with disulfide crosslinkages. These crosslinked fibers were easily decomposed under physiological conditions using L ‐cysteine, a biocompatible reductant. In vitro experiments with mouse L929 fibroblasts showed good adhesion onto γ ‐PGA‐SS fiber matrices and excellent cell proliferation. These γ ‐PGA‐SS nonwovens can be used as novel biocompatible and biodegradable scaffolds with reduction‐responsiveness for biomedical or tissue engineering applications.