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Effect of Head Size in Head‐Tail‐Type Polycations on their in vitro Performances as Nonviral Gene Vectors
Author(s) -
Harada Atsushi,
Kawamura Masanori,
Kimura Yuka,
Takahashi Toshinari,
Kojima Chie,
Kono Kenji
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800314
Subject(s) - dendrimer , biophysics , chemistry , polylysine , in vitro , phase locked loop , materials science , polymer chemistry , biology , biochemistry , phase (matter) , organic chemistry
Three kinds of head‐tail‐type block copolymers composed of polyamidoamine (PAMAM) dendron heads and poly( L ‐lysine) (PLL) tail blocks (PAMAM dendron‐PLL), having PAMAM dendrons with different generations (G2.5‐PLL, G3.5‐PLL and G4.5‐PLL) were synthesized. Some of the dendron heads were located at polyplex surface, and G2.5‐PLL and G3.5‐PLL could form small polyplexes (less than 150 nm in size). G2.5‐PLL and G3.5‐PLL polyplexes were taken up into the cells more effectively. PAMAM dendron‐PLL that had a larger dendron head could show a more‐effective buffering effect. The in vitro performance of the PAMAM dendron‐PLL polyplexes was controlled by the balance of cellular uptake and endosomal escape by a buffering effect.