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Nickel‐Ion‐Mediated Control of the Stoichiometry of His‐Tagged Protein/Nanoparticle Interactions
Author(s) -
De Mrinmoy,
Rana Subinoy,
Rotello Vincent M.
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800289
Subject(s) - stoichiometry , biomolecule , nanoparticle , chemistry , fluorescence , nickel , combinatorial chemistry , nanotechnology , materials science , biochemistry , organic chemistry , physics , quantum mechanics
The interaction between synthetic materials and biomolecules plays an important role in biomedical and pathological sciences. An important issue in these interactions is control of stoichiometry. The interaction between NTA ligands and proteins with six consecutive His residues has been widely used for protein purification. Control of stoichiometry is an important issue in applying this recognition strategy to the creation of defined nanoparticle‐protein conjugates. In this communication we report the direct control of particle‐protein stoichiometry through variation of nickel chloride concentration, as demonstrated through fluorescence and gel electrophoresis.