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Functionalization of Multi‐Walled Carbon Nanotubes and their Application for Selective Isolation of Acidic Proteins
Author(s) -
Du Zhuo,
Yu YongLiang,
Wang JianHua
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800200
Subject(s) - reagent , adsorption , elution , chemistry , surface modification , sorption , carbon nanotube , chromatography , activated carbon , buffer (optical fiber) , materials science , nanotechnology , organic chemistry , telecommunications , computer science
A micro‐column packed with PDDA‐wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA‐MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 µL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6–120 µg, resulting in an enrichment factor of 14. A sampling frequency of 15 h −1 is achieved, along with a precision of 4.5% at 25 µg · mL −1 BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.