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Orientation‐Selective Incorporation of Transmembrane F 0 F 1 ATP Synthase Complex from Micrococcus luteus in Polymer‐Supported Membranes
Author(s) -
Tutus Murat,
Rossetti Fernanda F.,
Schneck Emanuel,
Fragneto Giovanna,
Förster Friedrich,
Richter Ralf,
Nawroth Thomas,
Tanaka Motomu
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800128
Subject(s) - micrococcus luteus , atp synthase , membrane , transmembrane protein , vesicle , chemistry , biophysics , biochemistry , enzyme , biology , receptor , escherichia coli , gene
We report the vectorial incorporation of a highly asymmetric F 0 F 1 ATP synthase complex from Micrococcus luteus into polymer‐supported membranes. Dynamic light scattering and cryo electron microscopy confirm that the use of weak surfactants (bile acid) allows for the non‐disruptive protein incorporation into lipid vesicles. Spreading of vesicles with ATP synthase onto a cellulose support results in a homogeneous distribution of proteins, in contrast to a patchy image observed on bare glass slides. The orientation of ATP synthase can be identified using an antibody to the ATP binding site as well as from topographic profiles of the surface. The method to “align” transmembrane proteins in supported membranes would open a possibility to quantify protein functions in biomimetic model systems.