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Evaluating the Ability of Polyhydroxyalkanoate Synthase Mutants to Produce P(3HB ‐co‐ 3HA) from Soybean Oil
Author(s) -
Tsuge Takeharu,
Yamamoto Tetsuya,
Yano Kotaro,
Abe Hideki,
Doi Yoshiharu,
Taguchi Seiichi
Publication year - 2009
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800118
Subject(s) - ralstonia , polyhydroxyalkanoates , cupriavidus necator , mutant , chemistry , pseudomonas , biochemistry , enzyme , food science , bacteria , gene , biology , genetics
Polyhydroxyalkanoate (PHA) synthase from Pseudomonas sp 61‐3 (PhaC1 Ps ) is able to synthesize P(3HB ‐co‐ 3HA), consisting of a 3HB unit and medium‐chain‐length 3HA units of 6–12 carbon atoms. Expression vectors encoding 76 PhaC1 Ps mutants with an amino acid replacement at position 130, 325, 477 or 481 were individually introduced into Ralstonia eutropha . The mutant enzyme genes were evaluated in terms of their abilities to synthesize P(3HB ‐co‐ 3HA) using soybean oil as a carbon source. 20 mutants showed significantly high accumulation levels of PHA exceeding 30 wt.‐% and as high as 57 wt.‐%. It was found that hydrophobic amino acids at the positions are more likely to enhance accumulation of PHA in R. eutropha .