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Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression
Author(s) -
Cottenye Nicolas,
Teixeira Francisco,
Ponche Arnaud,
Reiter Günter,
Anselme Karine,
Meier Wolfgang,
Ploux Lydie,
VebertNardin Corinne
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200800081
Subject(s) - biofilm , oligonucleotide , adhesion , escherichia coli , wetting , chemistry , fluorescence microscope , biophysics , contact angle , covalent bond , bacteria , atomic force microscopy , x ray photoelectron spectroscopy , chemical engineering , materials science , fluorescence , nanotechnology , biochemistry , biology , dna , organic chemistry , gene , genetics , physics , engineering , quantum mechanics
Abstract Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide‐based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide‐modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria.