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Synthesis of Fluorescent Polyisoprene Nanoparticles and their Uptake into Various Cells
Author(s) -
Lorenz Myriam R.,
Kohnle MariaVerena,
Dass Martin,
Walther Paul,
Höcherl Anita,
Ziener Ulrich,
Landfester Katharina,
Mailänder Volker
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700336
Subject(s) - polystyrene , miniemulsion , nanoparticle , hela , internalization , biophysics , chemistry , fluorescence , intracellular , particle (ecology) , endosome , suspension (topology) , cell culture , cytoplasm , nanotechnology , polymer chemistry , materials science , cell , polymer , biochemistry , polymerization , organic chemistry , physics , oceanography , mathematics , genetics , quantum mechanics , homotopy , biology , geology , pure mathematics
Fluorescent polyisoprene nanoparticles were synthesized by the miniemulsion technique as marker particles for cells. The uptake of the non‐functionalized polyisoprene nanoparticles, without any transfection agents, into different adherent (HeLa) and also suspension (Jurkat) cell lines is strikingly efficient and fast compared to other polymeric particles, and leads to high loading of the cells. The intracellular polyisoprene particles are localized as single particles in endosomes distributed throughout the entire cytoplasm. The uptake kinetics shows that particle internalization starts during the first minutes of incubation and is finished after 48 h of incubation. Since (unfunctionalized) polystyrene particles show a comparable, low uptake behavior in cells, the uptake rates can be tuned by the amount of polystyrene in polyisoprene/polystyrene copolymer particles. As polyisoprene nanoparticles are internalized by different cell lines that are relevant for biomedical applications, they can be used to label these cells efficiently if a marker is incorporated in the particles. As polyisoprene is not or is hardly biodegradable the particles should be suited for long‐term applications.