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AFM Imaging of RGD Presenting Synthetic Extracellular Matrix Using Gold Nanoparticles
Author(s) -
Hsiong Susan X.,
Cooke Peter H.,
Kong HyunJoon,
Fishman Marshall L.,
Ericsson Maria,
Mooney David J.
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700313
Subject(s) - self healing hydrogels , biotinylation , extracellular matrix , adhesion , biomaterial , chemistry , colloidal gold , nanotechnology , atomic force microscopy , streptavidin , nanoparticle , biophysics , conjugated system , materials science , polymer , biochemistry , polymer chemistry , biotin , organic chemistry , biology
Several high‐resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High‐resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin‐labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.