Premium
Grafting BSA onto Poly[( L ‐lactide)‐ co ‐carbonate] Microspheres by Click Chemistry
Author(s) -
Han Yadong,
Shi Quan,
Hu Junli,
Du Qing,
Chen Xuesi,
Jing Xiabin
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700306
Subject(s) - chemistry , bovine serum albumin , polymer chemistry , grafting , lactide , click chemistry , carbonate , microsphere , covalent bond , fourier transform infrared spectroscopy , chemical engineering , nuclear chemistry , polymer , organic chemistry , chromatography , polymerization , engineering
Model protein bovine serum albumin (BSA) was covalently grafted onto poly[( L ‐lactide)‐ co ‐carbonate] microsphere surfaces by “click chemistry.” The grafting was confirmed by confocal laser scanning microscopy and X‐ray photoelectron spectroscopy. The maximum amount of surface‐grafted BSA was 45 mg · g −1 . The secondary structure of the grafted BSA was analyzed by FTIR and the results demonstrated that the grafting did not affect protein structure. This strategy can also be used on microspheres prepared from poly( L ‐lactide)/poly[( L ‐lactide)‐ co ‐carbonate] blend materials.