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Electrogeneration of Polypyrrole/Alginate Films for Immobilization of Glucose Oxidase
Author(s) -
Chen Shuangjun,
Chen Wei,
Xue Gi
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700273
Subject(s) - glucose oxidase , polypyrrole , covalent bond , immobilized enzyme , chemistry , substrate (aquarium) , electrochemistry , aqueous solution , polymer chemistry , electrode , enzyme , combinatorial chemistry , organic chemistry , oceanography , geology
Electrogenerated PPy doped with pSA was used as a substrate for immobilization of GOD. This was achieved via covalent bonding of carboxyl groups of the main chain of alginate with amino groups of the enzyme. The pH‐induced aggregation behavior of SA in aqueous solution was employed to provide optimum conditions for electrochemical preparation of PPy by galvanostatic methods. GOD was attached to the electrode surface by reaction between the carboxyl groups in the main chain of pSA with amino groups of GOD after treatment with EDC and NHS. The linkage of GOD enzyme to the conductive surface was characterized by ATR spectroscopy and SEM CV was used to demonstrate the bioactivity of the enzyme electrode toward glucose.