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Immobilization of Penicillin G Acylase on Poly[(glycidyl methacrylate)‐ co ‐(glycerol monomethacrylate)]‐Grafted Magnetic Microspheres
Author(s) -
Huang Junsheng,
Li Xiutao,
Zheng Yanhui,
Zhang Yong,
Zhao Ruiying,
Gao Xicai,
Yan Husheng
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700256
Subject(s) - glycidyl methacrylate , methacrylate , copolymer , monomer , polymer chemistry , polymer , thermal stability , chemistry , nuclear chemistry , materials science , chemical engineering , organic chemistry , engineering
Poly[(glycidyl methacrylate)‐ co ‐(glycerol monomethacrylate)]‐grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe 3 O 4 nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush‐grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/ glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.