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Biodegradable Nanoparticles of Partially Methylated Fungal Poly( β ‐ L ‐malic acid) as a Novel Protein Delivery Carrier
Author(s) -
PortillaArias José A.,
GarcíaAlvarez Montserrat,
Galbis Juan A.,
MuñozGuerra Sebastián
Publication year - 2008
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700249
Subject(s) - hydrolysis , chemistry , malic acid , nanoparticle , lipase , aqueous solution , polymer , degradation (telecommunications) , biodegradation , polymer chemistry , chemical engineering , organic chemistry , enzyme , telecommunications , computer science , citric acid , engineering
The preparation of nanoparticles from 75% methylated poly( β ‐ L ‐malic acid) is described. Their degradation in aqueous environments was examined and the influence of pH and lipase on the rate of hydrolysis was evaluated. Six proteins were used to estimate the loading efficiency of the nanoparticles. The amount of protein retained in the nanoparticles was found to depend on the acid/basic character of the protein. Protein release from the loaded nanoparticles upon incubation in water under physiological conditions encompassed polymer hydrolysis and happened steadily within 3–10 d. The activity loss of entrapped α ‐chymotrypsin caused by loading and releasing depended on the method used for loading.