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Premium Relationship between Enzyme Adsorption and Enzyme‐Catalyzed Degradation of Polylactides
Author(s)
Zhao Zhenxian,
Yang Liu,
Hua Jiajie,
Wei Jia,
Gachet Sylvie,
Ghzaoui Abdelslam El,
Li Suming
Publication year2008
Publication title
macromolecular bioscience
Resource typeJournals
PublisherWILEY‐VCH Verlag
Abstract The adsorption of proteinase K on PLLA and PDLA films was studied by CA, surface tension, and microscopic measurements. ESEM clearly shows that proteinase K can irreversibly adsorb on PLLA film. In contrast, no enzyme adsorption was detected on PDLA film under the same conditions. The CA of PLLA film rapidly decreases after immersion in Tris buffer containing proteinase K, whereas that of PDLA remains unchanged. These findings indicate that enzyme adsorption may be a prerequisite for enzymatic degradation of polylactide substrates. Surface tension measurements allow calculation of the average area occupied per proteinase K molecule. The results show that the enzyme molecules exhibit a more compact conformation at higher temperature.
Subject(s)adsorption , biochemistry , biology , biophysics , catalysis , chemical engineering , chemistry , computer science , degradation (telecommunications) , engineering , enzyme , molecule , organic chemistry , physics , surface tension , telecommunications , thermodynamics , tris
Language(s)English
SCImago Journal Rank0.924
H-Index105
eISSN1616-5195
pISSN1616-5187
DOI10.1002/mabi.200700180

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