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Cellular Uptake Behavior of Unfunctionalized and Functionalized PBCA Particles Prepared in a Miniemulsion
Author(s) -
Weiss Clemens K.,
Lorenz Myriam R.,
Landfester Katharina,
Mailänder Volker
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700046
Subject(s) - miniemulsion , surface modification , molar mass , chemistry , jurkat cells , hela , polymer chemistry , nanoparticle , polymer , particle size , polymerization , emulsion polymerization , chemical engineering , nuclear chemistry , nanotechnology , materials science , organic chemistry , biochemistry , cell , immune system , t cell , engineering , immunology , biology
Fluorescent dye labeled unfunctionalized and functionalized poly( n ‐butylcyanoacrylate) nanoparticles were prepared using a miniemulsion technique. Amino acid and methoxyPEG functionalization could be introduced by using aqueous solutions as an initiator for the anionic polymerization in the heterophase. All the particles prepared had sizes smaller than 250 nm and negative ζ ‐potentials. The molar mass distribution of the polymer was dependent on the acid used as the continuous phase and the initiator solution applied. Cells of three lines (HeLa, Jurkat and mesenchymal stem cells) were incubated with the particles. The molar mass of the polymer determined the onset and extent of apoptosis, and the total uptake was determined by the size and functionalization of the particles. Different uptake kinetics were obtained with HeLa and Jurkat cells after incubation with the same particle batch. The intracellular particle distribution, visualized by confocal laser scanning microscopy, did not show significant differences for either of the cell lines or particle batches.