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Ferrocene Branched Chitosan for the Construction of a Reagentless Amperometric Hydrogen Peroxide Biosensor
Author(s) -
Garcia Ariel,
PenicheCovas Carlos,
Chico Belkis,
Simpson Benjamin K.,
Villalonga Reynaldo
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700032
Subject(s) - ferrocene , amperometry , hydrogen peroxide , biosensor , chemistry , horseradish peroxidase , chitosan , immobilized enzyme , inorganic chemistry , electrochemistry , nuclear chemistry , electrode , organic chemistry , enzyme , biochemistry
Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 × 10 −6 M and 2.0 × 10 −3 M . The biosensor reached 95% of the steady‐state current in about 20 s and its sensitivity was 28.4 × 10 −3 µA · M −1 . The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 °C in 50 × 10 −3 M sodium phosphate buffer at pH 7.0.