Premium
Direct Transfer of Preformed Patterned Bio‐Nanocomposite Films on Polyelectrolyte Multilayer Templates
Author(s) -
Kohli Neeraj,
Worden Robert M.,
Lee Ilsoon
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200700006
Subject(s) - polyelectrolyte , nanotechnology , template , materials science , biosensor , substrate (aquarium) , self assembly , protein microarray , dendrimer , adhesion , fabrication , polymer , dna microarray , chemistry , polymer chemistry , medicine , biochemistry , oceanography , gene expression , alternative medicine , pathology , geology , composite material , gene
Microarrays containing multiple, nanostructured layers of biological materials would enable high‐throughput screening of drug candidates, investigation of protein‐mediated cell adhesion, and fabrication of novel biosensors. In this paper, we have examined in detail an approach that allows high‐quality microarrays of layered, bionanocomposite films to be deposited on virtually any substrate. The approach uses LBL self‐assembly to pre‐establish a multilayered structure on an elastomeric stamp, and then uses µCP to transfer the 3‐D structure intact to the target surface. For examples, different 3‐D patterns containing dendrimers, polyelectrolyte multilayers and two proteins, sADH and sDH, have been fabricated. For the first time, the approach was also extended to create overlaid bionanocomposite patterns and multiple proteins containing patterns. The approach overcomes a problem encountered when using µCP to establish a pattern on the target surface and then building sequential layers on the pattern via LBL self‐assembly. Amphiphilic molecules such as proteins and dendrimers tend to adsorb both to the patterned features as well as the underlying substrate, resulting in low‐quality patterns. By circumventing this problem, this research significantly extends the range of surfaces and layering constituents that can be used to fabricate 3‐D, patterned, bionanocomposite structures.