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Human Hepatocyte Morphology and Functions in a Multibore Fiber Bioreactor
Author(s) -
De Bartolo Loredana,
Morelli Sabrina,
Rende Maria,
Campana Carla,
Salerno Simona,
Quintiero Nino,
Drioli Enrico
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600281
Subject(s) - bioreactor , hepatocyte , bioartificial liver device , fiber , in vitro , hollow fiber membrane , human liver , in vivo , chemistry , microbiology and biotechnology , membrane , biochemistry , biology , organic chemistry
The viability and liver specific functions of human hepatocytes in a multibore fiber bioreactor are reported. Human hepatocytes were cultured in the intraluminal compartment of the bioreactor. Human hepatocytes on the membranes maintained their round shape and showed focal adhesions as sites of interaction with the membrane surface. Cells in the bioreactor expressed liver specific functions, including synthetic and detoxification activity up to 14 d of culture. The results demonstrate that human hepatocytes cultured in the multibore fiber bioreactor are able to sustain the same in vivo liver functions in vitro .