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Improved Mesenchymal Stem Cell Seeding on RGD‐Modified Poly( L ‐lactic acid) Scaffolds using Flow Perfusion
Author(s) -
AlvarezBarreto Jose F.,
Sikavitsas Vassilios I.
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600280
Subject(s) - seeding , mesenchymal stem cell , chemistry , biomedical engineering , perfusion , tissue engineering , cell , biophysics , adhesion , scaffold , cell adhesion , cell culture , microbiology and biotechnology , biochemistry , biology , medicine , genetics , organic chemistry , agronomy , cardiology
Arg‐Gly‐Asp (RGD) has been widely utilized to increase cell adhesion to three‐dimensional scaffolds for tissue engineering. However, cell seeding on these scaffolds has only been carried out statically, which yields low cell seeding efficiencies. We have characterized, for the first time, the seeding of rat mesenchymal stem cells on RGD‐modified poly( L ‐lactic acid) (PLLA) foams using oscillatory flow perfusion. The incorporation of RGD on the PLLA foams improves scaffold cellularity in a dose‐dependent manner under oscillatory flow perfusion seeding. When compared to static seeding, oscillatory flow perfusion is the most efficient seeding technique. Cell detachment studies show that cell adhesion is dependent on the applied flow rate, and that cell attachment is strengthened at higher levels of RGD modification.