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Covalent Grafting of Fibronectin onto Plasma‐Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity
Author(s) -
Vallières Karine,
Petitclerc Éric,
Laroche Gaétan
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600267
Subject(s) - fibronectin , covalent bond , chemistry , biocompatibility , grafting , adhesion , polyclonal antibodies , polymer chemistry , coating , fibronectins , cell adhesion , biophysics , biochemistry , extracellular matrix , antibody , organic chemistry , polymer , immunology , biology
Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐( p ‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.