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Evaluation of Endothelial Cell Adhesion onto Different Protein/Gold Electrodes by EIS
Author(s) -
Bouafsoun Amira,
Helali Saloua,
Othmane Ali,
Kerkeni Abdelhamid,
Prigent AnneFrance,
JaffrézicRenault Nicole,
Bessueille François,
Léonard Didier,
Ponsonnet Laurence
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600263
Subject(s) - chemistry , adhesion , cell adhesion , protein adsorption , biophysics , fibronectin , dielectric spectroscopy , biomolecule , albumin , cytoskeleton , cell , adsorption , biochemistry , electrode , electrochemistry , organic chemistry , biology
To study cell attachment to biomaterials, several proteins such as fibronectin, collagen IV, heparin, immunoglobulin G, and albumin have been deposited onto polystyrene adsorbed on a self‐assembled monolayer (silane or thiol) on glass or gold, respectively. The different steps of this multilayer assembly have been characterized by electrochemical impedance spectroscopy (EIS). These data are compared to those of adhesion rate, viability percentage, and cytoskeleton labeling for a better understanding of the cell adhesion process to each protein. All the proteins are endothelial cell adhering biomolecules but not with the same features. A linear relationship has been established between adhesion rate and resistance of the endothelial cell/protein interface for all negatively charged proteins.