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A Degradable Hyperbranched Poly(ester amine) Based on Poloxamer Diacrylate and Polyethylenimine as a Gene Carrier
Author(s) -
Kim Tae Hee,
Cook Seung Eun,
Arote Rohidas B.,
Cho MyungHaing,
Nah Jae Woon,
Choi Yun Jaie,
Cho Chong Su
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600245
Subject(s) - polyethylenimine , poloxamer , gene delivery , chemistry , cytotoxicity , transfection , cationic polymerization , polymer chemistry , monomer , polymer , a549 cell , biophysics , amine gas treating , cell culture , organic chemistry , biochemistry , in vitro , gene , copolymer , biology , genetics
Polyethylenimine (PEI) is a well‐known cationic polymer which has high transfection efficiency due to its buffering effect. However, nondegradability, cytotoxicity, aggregation, and short‐circulation time in vivo still need to be overcome for a successful gene delivery. Degradable, hyperbranched poly(ester amine)s (PEAs) based on poloxamer diacrylate and low molecular weight branched PEI, were successfully synthesized and evaluated as a nonviral gene carrier. The PEAs were obtained in significant yields through Michael type addition reaction of diacrylate monomers and low molecular weight branched PEI. Analysis of degradation products by the reduction in molecular weight demonstrated that PEAs degrade in a controlled fashion. The PEA showed good DNA binding ability and the sizes of complexes under physiological condition were below 150 nm, implicating its potential for intracellular delivery. It showed lower cytotoxicity in three different cell lines (A549, 293T, and HepG2) compared with PEI 25K. PEAs showed much higher transfection efficiencies in three cell lines compared with PEI 25K and PEI 1.8K, and revealed little serum dependency in A549 cell line when the content of poloxamer in the PEA was increased up to 30%.