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Preparation and Characterization of a Scaffold for Vascular Tissue Engineering by Direct‐Assembling of Collagen and Cells in a Cylindrical Geometry
Author(s) -
Boccafoschi Francesca,
Rajan Navneeta,
Habermehl Jason,
Mantovani Diego
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600242
Subject(s) - scaffold , tissue engineering , biomedical engineering , scanning electron microscope , extracellular matrix , matrix (chemical analysis) , materials science , chemistry , anatomy , nanotechnology , composite material , biology , engineering , biochemistry
In the past 25 years, several efforts have been focused on developing vascular substitutes showing long‐term patency when implanted in humans. In this study, we present our last findings in an ongoing project aiming to develop a tissue‐regenerated blood vessel from collagen‐based scaffolds. A collagen‐based scaffold with adequate biological properties was developed by directly assembling collagen and cells in a cylindrical geometry. The technique mainly involves the use of a ventilated rotating tube allowing smooth muscle cells to grow in a cylindrical collagen matrix. Scanning electron microscopy (SEM), histology, and cell cycle analyses were carried out in order to assess the biological potential of the cell‐based scaffold for further maturation. Compliance results showed that this technique allows cells to assemble in the collagen matrix, thus providing enough rigidity to the structure to be handled and mounted in a perfusion bioreactor for further growth and maturation.