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Biosynthesis and Biodegradability of Copolythioesters from 3,3′‐Thiodipropionic Acid and Plant Oils by Cupriviadus necator
Author(s) -
Kamei Yasutaka,
Nagai Atsushi,
Nishida Haruo,
Kimura Hiroshi,
Endo Takeshi
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600239
Subject(s) - cupriavidus necator , biodegradation , copolymer , chemistry , hydrolysis , biopolymer , polymer , degradation (telecommunications) , biosynthesis , catalysis , bacteria , organic chemistry , polymer chemistry , polyhydroxyalkanoates , enzyme , biology , computer science , telecommunications , genetics
To prepare sulfur‐containing natural polymers effectively, several plant oils and 3,3′‐thiodipropionic acid (TDP) have been used as carbon sources for the biosynthesis of copolymer poly[(3‐hydroxybutyrate)‐ co ‐(3‐mercaptopropionate)] [poly(3HB‐ co ‐3MP)] by a wild‐type bacterium Cupriviadus necator H16. By using the plant oils, copolymer accumulation and incorporation of 3MP units are greater than those of cases using sugars. The 3MP fraction is controllable over a range of 1–39 mol‐% by adjusting the cultivation conditions. Microbial degradability of the copolymers has been examined in an activated sludge supernatant. The biodegradation proceeded by two mechanisms: surface erosion and auto‐catalytic hydrolysis, depending on the 3MP unit fraction, and show preferential degradation of 3HB unit sequences.