Premium
Increase of Fluorescence Anisotropy Upon Self‐Assembly in Headgroup‐Labeled Surfactants
Author(s) -
Kastantin Mark,
Ananthanarayanan Badriprasad,
Lin Brian,
Ressl Jan,
Black Matthew,
Tirrell Matthew
Publication year - 2007
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600203
Subject(s) - fluorescence anisotropy , fluorophore , chemistry , anisotropy , fluorescence , micelle , vesicle , pulmonary surfactant , rotational correlation time , chemical physics , membrane , analytical chemistry (journal) , aqueous solution , molecule , organic chemistry , optics , biochemistry , physics
The change in fluorescence anisotropy upon micellization in headgroup‐labeled surfactants is investigated. After eliminating the likelihood of depolarizing RET, anisotropy is shown to increase upon self‐assembly due to increased rotational correlation times of the fluorophore. This is shown using two surfactant‐fluorophore systems. Anisotropy in NBD‐labeled phospholipids is studied both in chloroform (unaggregated) and in water (unilamellar vesicles), while in tryptophan‐containing peptide‐amphiphiles, the variation of anisotropy with concentration leads to a reasonable measurement of CAC. Anisotropy increase is shown to be largely the product of increased rotational correlation times for the fluorophore, relative to its τ . These results serve as a basis for future work that measures the amount of depolarizing energy transfer, characterizing distances between similar fluorescent headgroups on mixed micelles.