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Surface Behavior and Molecular Recognition in DNA Microarrays from N , N ‐Dimethylacrylamide Terpolymers with Activated Esters as Linking Groups
Author(s) -
Suriano Raffaella,
Levi Marinella,
Pirri Giovanna,
Damin Francesco,
Chiari Marcella,
Turri Stefano
Publication year - 2006
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200600088
Subject(s) - contact angle , copolymer , polymer chemistry , chemical engineering , materials science , surface modification , ellipsometry , chemistry , fluorescence , polymer , analytical chemistry (journal) , nanotechnology , thin film , organic chemistry , composite material , engineering , physics , quantum mechanics
Summary: A series of terpolymers made of DMA, NAS and MAPS were synthesized by free radical copolymerization and used as functional coatings for the fabrication of glass slide DNA microarrays. The surface properties of coated glass slides were investigated through contact angle measurements, ellipsometry and atomic force microscopy. The terpolymer molecular weight showed a moderate effect on surface tension ( γ s = 56–62 mN · m −1 ), but no clear effect on polymeric layer thickness (5–8 nm) and roughness. Hybridization experiments with amine‐functionalized oligonucleotides gave the best fluorescence intensity results for microarrays coated with intermediate‐molecular‐weight terpolymers. Finally, an accelerated ageing test of the microarray in a humidity chamber showed a nice relationship between decay curves of contact angle against water and fluorescence intensity.Details of fluorescence microscope images after hybridization of the probe H 2 N‐oligonucleotide spotted on the polymeric coatings.