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Biodegradable Hyaluronic Acid/ N ‐Carboxyethyl Chitosan/Protein Ternary Complexes as Implantable Carriers for Controlled Protein Release
Author(s) -
Jiang Hongliang,
Wang Yijuan,
Huang Qian,
Li Yan,
Xu Chaonan,
Zhu Kangjie,
Chen Weiliam
Publication year - 2005
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.200500126
Subject(s) - chemistry , chitosan , isoelectric point , ionic strength , titration , bovine serum albumin , hyaluronic acid , nuclear chemistry , solubility , ternary complex , chromatography , polymer chemistry , aqueous solution , organic chemistry , enzyme , biology , genetics
Summary: An ampholytic N ‐carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high‐MW HA and CEC with low DS.BSA release profiles from HA/CEC‐2/BSA complexes.